Composite

Part:BBa_K2448054

Designed by: Ioana Popescu   Group: iGEM17_Evry_Paris-Saclay   (2018-12-27)


D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum with a C-terminal HisTag under the contr

This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944) with a C-terminal HisTag (BBa_K2448053) equipped with a strong RBS (BBa_K2448019) and placed under the control of the pTacI promoter (BBa_K864400).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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